Sir William Wightman was a British judge.
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{"type":"standard","title":"Fura-2-acetoxymethyl ester","displaytitle":"Fura-2-acetoxymethyl ester","namespace":{"id":0,"text":""},"wikibase_item":"Q424893","titles":{"canonical":"Fura-2-acetoxymethyl_ester","normalized":"Fura-2-acetoxymethyl ester","display":"Fura-2-acetoxymethyl ester"},"pageid":4128785,"thumbnail":{"source":"https://upload.wikimedia.org/wikipedia/commons/thumb/4/49/Fura_2AM.png/330px-Fura_2AM.png","width":320,"height":320},"originalimage":{"source":"https://upload.wikimedia.org/wikipedia/commons/4/49/Fura_2AM.png","width":909,"height":910},"lang":"en","dir":"ltr","revision":"1248796086","tid":"3d63a9bb-8002-11ef-b107-e63b2fb7d9b7","timestamp":"2024-10-01T14:34:18Z","description":"Chemical compound","description_source":"local","content_urls":{"desktop":{"page":"https://en.wikipedia.org/wiki/Fura-2-acetoxymethyl_ester","revisions":"https://en.wikipedia.org/wiki/Fura-2-acetoxymethyl_ester?action=history","edit":"https://en.wikipedia.org/wiki/Fura-2-acetoxymethyl_ester?action=edit","talk":"https://en.wikipedia.org/wiki/Talk:Fura-2-acetoxymethyl_ester"},"mobile":{"page":"https://en.m.wikipedia.org/wiki/Fura-2-acetoxymethyl_ester","revisions":"https://en.m.wikipedia.org/wiki/Special:History/Fura-2-acetoxymethyl_ester","edit":"https://en.m.wikipedia.org/wiki/Fura-2-acetoxymethyl_ester?action=edit","talk":"https://en.m.wikipedia.org/wiki/Talk:Fura-2-acetoxymethyl_ester"}},"extract":"Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeant derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates \"Fura-2\", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium concentrations in cells.","extract_html":"
Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeant derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates \"Fura-2\", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium concentrations in cells.
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